تکثیر درون شیشه‌ای نیشکر (Saccharum officinarum)

Experiments were carried out in order to micropropagate sugarcane cultivars through shoot tip and auxilliary bud culture. Rinsing of four cultivars of sugarcane, namely CP-48-103, CP-57-614, CP-69-1062, and NCO-310 in 75% alcohol for 60 seconds and their subsequent disinfection with sodium and calcium hypochloride (1.5% active material) for 15 minutes decreased a significant amount of infection of explants in the medium. The use of the Murashing and Skoog (MS) solid and liquid medium with 1 mg/l Indole Butyric Acid (IBA), 1 mg/l Kinetin, 100 mg/l mio-inositol, 1 mg/l Thiamin HCl, and 2% sucrose had significant superiority (P<0.05) to 1/2 MS solid medium. Also, to increase the multiplication in a sterile medium (In vitro), two kinds of solid and liquid MS medium, with a hormone combination of 1 mg/l IBA, 2 mg/l Kinetin and 1 mg/l 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (BAP) were applied which yielded the highest amount of proliferation. The plants formed roots in Schenk and Hildebrandt (SH) medium with a hormone combination of 5 mg/l IBA and 1 mg/l Kinetin. When activate charcoal was used in the medium, a higher percentage of the plants became rooted and a larger number of adventitious roots were produced than in the dark-light or light treatments.